Neymar could be an injury doubt for Paris Saint-Germain's Champions League clash with Liverpool next week after limping off during Brazil's 1-0 friendly win over Cameroon on Tuesday.
The forward suffered an apparent groin problem and was replaced in the eighth minute, to boos from the crowd in the central English town of Milton Keynes.
Everton's Richarlison, Neymar's replacement, headed in on the stroke of half-time for his third Brazil goal on his sixth appearance to secure victory for the Selecao.
A mixture of cheers and jeers welcomed Neymar, his World Cup theatrics in Russia providing ammunition for his detractors.
And there was little sympathy when the star attraction went off after making little impact.
He sought treatment after five minutes and returned to send a curling right-footed shot high and wide within a minute. He immediately collapsed before being replaced.
Meanwhile, A shoulder injury to Kylian Mbappe overshadowed France's 1-0 friendly win over Uruguay at the Stade de France on Tuesday as Didier Deschamps' world champions ended the year on a high note.
Mbappe had to be replaced in the first half after a nasty fall, continuing a miserable night for his Paris Saint-Germain coach Thomas Tuchel after the German had already seen Neymar hobble off injured early in Brazil's friendly with Cameroon in Milton Keynes.
After the disappointment of seeing the Netherlands pip them to a place at the Nations League finals with a late Virgil van Dijk goal in Germany on Monday, Olivier Giroud's 52nd-minute penalty gave France victory in a repeat of the two teams' World Cup quarter-final meeting in Russia.
Want stories like this in your inbox?
Sign up to exclusive daily email
More Stories from Football